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December 8, 2025BloodOpen Access

Detecting lineage-specific regulatory activity and stemness retention in single-cell multiomics of human hematopoiesis

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Authors

CJC. Diana JordanDODominik J. OttoMSManu Setty

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Overview

Single-cell multiomics reveals epigenetic influences on cell fate in hematopoietic stem cells, indicating implications for hematologic malignancies.

Key Points

  • This research aims to understand how epigenetic regulator mutations influence hematopoietic stem and progenitor cells during human hematopoiesis.
  • Utilized single-cell multi-omics data from healthy human bone marrow
  • Processed scATAC-seq and sciCUTAC data using Seurat and Signac
  • Constructed pseudotime axis with Palantir
  • Computed gene scores for gene level analyses using ArchR
  • Fitted regulatory activity trends with Gaussian process regression
  • Identified lineage-specific regulatory dynamics affecting HSPC fate decisions
  • Observed co-occurring chromatin changes at transcription factor loci in B and myeloid lineages
  • Demonstrated patterns of epigenetic mark enrichment associated with key regulators like PAX5 and EBF1
  • Erased stem cell program as cells transition through fate alterations

Cite This Study

Jordan et al. (2025) studied this question.

synapsesocial.com/papers/69362f484fa91c937236d6d7https://doi.org/10.1182/blood-2025-6119
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