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December 8, 2025Blood

Trimodal single-cell profiling reveals principles of cell fate acquisition in human erythropoiesis.

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Authors

STSteven TurJRJeffrey A. Ranish

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Overview

Trimodal assay shows dynamic chromatin remodeling and transcriptional shifts in hematopoiesis, indicating complex gene regulatory networks.

Key Points

  • This research aims to understand the principles of cell fate acquisition during human erythropoiesis.
  • Applied trimodal single-cell assay TEA-seq to profile gene expression, chromatin accessibility, and surface proteins in individual cells.
  • Isolated hematopoietic stem and progenitor cells from umbilical cord blood and collected data across four time points in a fourteen-day erythroid differentiation culture.
  • Integrated data using MultiVI, a probabilistic model for shared latent space.
  • Established a high-resolution map of human hematopoiesis linking cell fate acquisition with chromatin and protein signatures.
  • Identified that transcription factor abundance drives early changes, while chromatin remodeling dominates intermediate stages, and transcriptional dynamics regain precedence later.
  • Mapped dynamic rewiring of gene regulatory networks aligning with differentiation trajectories and revealing active stemness-related networks.

Cite This Study

Tur et al. (2025) studied this question.

synapsesocial.com/papers/69362f764fa91c937236e361https://doi.org/10.1182/blood-2025-967
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  1. 1Trimodal Single-Cell Gene Regulatory Networks Reveal Principles of Stemness Loss and Cell Fate Acquisition in Human Hematopoiesis2025 · 1 citations
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  5. 5Chromatin Accessibility Shapes Developmental-Specific Lineage Plasticity in Hematopoiesis2026