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December 8, 2025BloodOpen Access

Generation of iNKT cells with hematopoiesis-promoting properties from human iPSCs

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Authors

AKAkhilesh KumarJGJenny E. Gumperz

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Overview

Laboratory-generated iNKT cells enhance hematopoiesis in cord blood, suggesting potential for stem cell therapies.

Key Points

  • This research aims to explore the potential of human iPSC-derived iNKT cells in promoting hematopoiesis.
  • Reprogrammed human CD4+Vα24+ iNKT cells into induced pluripotent stem cells (iPSCs).
  • Developed a feeder-free 3D spheroid culture method to generate CD34+ hematopoietic progenitors.
  • Cultured floating hematopoietic progenitors and activated them on OP9-DLL4 cultures for lymphocyte generation.
  • Generated i-iNKT cells expressed similar transcription factors to somatic iNKT cells.
  • Demonstrated production of GM-CSF, IL-3, IFN-g, and IL-13 upon stimulation, but no IL-4.
  • i-iNKT cells significantly enhanced differentiation of CD34+ cord blood cells.

Cite This Study

Kumar et al. (2025) studied this question.

synapsesocial.com/papers/69362f4e4fa91c937236d877https://doi.org/10.1182/blood-2025-4184
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Also Consider

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  1. 1Generation of iPSC-Derived iNKT Cells with Pro-Hematopoietic Activity2025
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  4. 4A scalable, spin‐free approach to generate enhanced induced pluripotent stem cell–derived <scp>natural killer</scp> cells for cancer immunotherapy2024 · 9 citations
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