What question did this study set out to answer?

To explore the role of mitochondrial function in neutrophil overactivation during sepsis and assess the effects of BAM15.

December 8, 2025BloodOpen Access

Targeting mitochondrial metabolism to suppress neutrophil overactivation in sepsis: Therapeutic potential of the mitochondrial uncoupler BAM15

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This study demonstrates mitochondrial uncoupler BAM15 reduces neutrophil overactivation in sepsis, suggesting new anti-inflammatory strategies.

To explore the role of mitochondrial function in neutrophil overactivation during sepsis and assess the effects of BAM15.
Isolated neutrophils from patients with sepsis and healthy controls via density gradient centrifugation.
Assessed mitochondrial respiration and glycolytic rate using Seahorse analysis and stress test kits.
Measured mitochondrial membrane potential and ROS using flow cytometry with specific dyes.
Conducted functional tests for chemotaxis, phagocytosis, and NET formation using various assays.
Neutrophils from sepsis patients showed an activated phenotype with increased CD11b and reduced CD62L.
Sepsis neutrophils displayed elevated oxygen consumption and extracellular acidification rates compared to controls.
BAM15 treatment reduced mitochondrial membrane potential and mtROS levels, attenuating excessive neutrophil functions.

Charoensappakit et al. (2025) studied this question.

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This study demonstrates mitochondrial uncoupler BAM15 reduces neutrophil overactivation in sepsis, suggesting new anti-inflammatory strategies.