What type of study is this?

This is a In Vitro Study study.

What question did this study set out to answer?

The aim is to investigate the second-hit effect of extracellular vesicles on heme-primed endothelial cells in Sickle cell disease.

December 8, 2025BloodOpen Access

In vitro microfluidics model revealed red blood cell derived extracellular vesicles cause second hit to heme-primed endothelial cells

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Authors

QHQingrong HeUniversity of Houston CLChang LiuHelmholtz Institute Jena

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Implication

In vitro model shows red blood cell derived extracellular vesicles enhance endothelial activation in Sickle cell disease, indicating additional factors are involved.

Key Points

The aim is to investigate the second-hit effect of extracellular vesicles on heme-primed endothelial cells in Sickle cell disease.
Established an in vitro microfluidic model using SCD-EV-BioChip.
Evaluated REV-mediated endothelial cell activation using THP-1 monocyte adhesion as a biomarker.
Measured heme content and prepared heme-primed HUVECs for experiments.
Conducted complementary activation assessments using ELISA for C5a levels.
SCD REVs and heme enhanced complement system activation.
Monocyte adhesion was highest in endothelial cells primed with heme followed by REVs.
Revs caused greater adhesion compared to sequential heme treatments alone.

Cite This Study

He et al. (2025) studied this question.

synapsesocial.com/papers/69362f5a4fa91c937236dadd https://doi.org/10.1182/blood-2025-4711

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Implication

In vitro model shows red blood cell derived extracellular vesicles enhance endothelial activation in Sickle cell disease, indicating additional factors are involved.

In vitro microfluidics model revealed red blood cell derived extracellular vesicles cause second hit to heme-primed endothelial cells

Discussion

Member takes

Implication

Key Points

Cite This Study

In vitro microfluidics model revealed red blood cell derived extracellular vesicles cause second hit to heme-primed endothelial cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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