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December 8, 2025Blood

Single-cell genotyping and methylome sequencing (scGEM-seq) resolves cell-states and clonal hierarchies in leukemia

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KKKyung Lock Kim

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Overview

Novel platform integrates genetic variants and methylation profiles in leukemia, suggesting improved detection of minimal residual disease.

Key Points

  • This research aims to develop a flexible and cost-effective platform for analyzing genetic and epigenetic factors in leukemia.
  • Developed scGEM-seq platform for simultaneous genetic and epigenetic analysis.
  • Utilized single-cell capture with semi-permeable capsules and HexaDecimal barcoding for unique cell identification.
  • Analyzed primary bone marrow samples alongside mixed cancer cell lines for genotyping efficiency.
  • Successfully resolved distinct cell populations in leukemia based on genetics and methylation profiles.
  • Achieved consistent single-cell genotyping for known cancer genes across various cell lines.
  • Identified subclonal and clonal malignant cells in primary samples with specific mutations.

Cite This Study

Kyung Lock Kim (2025) studied this question.

synapsesocial.com/papers/69362f604fa91c937236dcafhttps://doi.org/10.1182/blood-2025-942
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