Single-cell RNA sequencing reveals a unique Th1-like T-cell population in immune thrombocytopenia, suggesting tacrolimus may remodel T-cell dysfunction.
Key Points
To investigate the pathogenic T-cell response and the effects of tacrolimus in immune thrombocytopenia.
Isolated peripheral blood mononuclear cells (PBMCs) from ITP patients and healthy controls.
Applied single-cell RNA sequencing to analyze T-cell subsets.
Employed pathway enrichment analysis to assess gene expression patterns.
Identified a distinct Th1-like CD4 T-cell subset in ITP patients.
Found that CXCR3+ T-cells were linked to lower platelet counts.
Tacrolimus treatment resulted in a decreased frequency of the pathogenic T-cell subset, normalizing expression features.