What question did this study set out to answer?

To evaluate the effectiveness of 5-hydroxymethylfurfural on red blood cell deformability in sickle cell disease using Brillouin microscopy.

December 8, 2025BloodOpen Access

Contact-free single cell assay for assessing RBC mechanical properties using brillouin microscopy: Evaluation of anti-sickling therapeutics

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Brillouin microscopy demonstrates improvements in RBC deformability with 5-HMF in sickle cell disease, indicating potential therapeutic benefits.

To evaluate the effectiveness of 5-hydroxymethylfurfural on red blood cell deformability in sickle cell disease using Brillouin microscopy.
Developed a contact-free assay using Brillouin microscopy to image single RBCs.
Sickle RBCs were isolated using percoll-based density gradient centrifugation and treated with varying concentrations of 5-HMF.
Assessed Brillouin frequency shift under controlled oxygen conditions to measure RBC deformability.
Conducted statistical analyses with GraphPad Prism software.
Significant reduction in Brillouin frequency shift indicates improved RBC deformability at 2.5 mM and 5 mM 5-HMF doses.
Point of sickling and recovery showed significant improvements at higher 5-HMF concentrations, correlating with enhanced biomechanics.
Optimal deformability observed at 2.5 mM 5-HMF across varying oxygenation levels.

Anand et al. (2025) studied this question.

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Brillouin microscopy demonstrates improvements in RBC deformability with 5-HMF in sickle cell disease, indicating potential therapeutic benefits.