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December 8, 2025BloodOpen Access

Novel microfluidic rheology assay for in vitro-differentiated red blood cells: Enhancing quality control and therapeutic evaluation in sickle cell disease gene therapies

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Authors

YDYong DuYLYusang M. Bejarano LeyNPN Pérez

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Overview

Innovative assay demonstrates evaluation of red blood cells in sickle cell disease, indicating improved quality control and gene therapy assessment.

Key Points

  • This research aims to develop a microfluidic rheology assay for quality control and evaluation of in vitro-differentiated red blood cells in sickle cell disease gene therapies.
  • Developed a microfluidic assay for rheological analysis of red blood cells
  • Analyzed nucleated and enucleated cell populations
  • Utilized computer vision for tracking RBC velocities
  • Tested mixed samples of healthy and sickle cell disease RBCs
  • Evaluated slow RBC fraction as a key metric.
  • Healthy RBCs exhibited a slow fraction of 19.0% compared to 42.1% in sickle cell disease RBCs
  • Enriched enucleated cells showed similar velocity profiles to healthy RBCs
  • Detected subtle rheological abnormalities in mixed RBC samples

Cite This Study

Du et al. (2025) studied this question.

synapsesocial.com/papers/69362f744fa91c937236e2d4https://doi.org/10.1182/blood-2025-6489
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sickling kinetics drive genotype-specific impairment of RBC deformability in sickle cell disease2025
  2. 2Microfluidic capillary transit velocity as a functional measure for sickle cell disease and in vitro-derived red blood cells2026
  3. 3Blood Rheology After Allogeneic Hematopoietic Stem Cell Transplantation or Gene Therapy in Sickle Cell Disease2026
  4. 4A framework of computer vision-enhanced microfluidic approach for automated assessment of the transient sickling kinetics in sickle red blood cells2024 · 11 citations
  5. 5A Microfluidic Approach for Assessing the Rheological Properties of Healthy Versus Thalassemic Red Blood Cells2025 · 7 citations