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The study aims to assess the changes in donkey milk's chemical composition and bioactive protein profile across lactation.

December 12, 2025FoodsOpen Access

Bioactive Protein Profile and Compositional Evolution of Donkey Milk Across Lactation Reflecting Its Nutritional and Functional Food Value

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Observational study shows changes in bioactive proteins and composition in donkey milk during lactation, indicating nutraceutical benefits.

The study aims to assess the changes in donkey milk's chemical composition and bioactive protein profile across lactation.
Longitudinal observational study
153 donkey milk samples from five farms
Chemical composition analyzed using mid-infrared spectroscopy
Immune-active proteins quantified by ELISA Quantitative Sandwich
Total solids and protein in donkey milk decline from early to late lactation
Lactoferrin concentration increases over lactation
α-lactalbumin peaks mid-lactation, while β-lactoglobulin and lysozyme decline
Stable levels of lactose and modest variation in fat observed
Findings support the nutraceutical relevance of donkey milk for human nutrition.

Plotuna et al. (2025) studied this question.

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Observational study shows changes in bioactive proteins and composition in donkey milk during lactation, indicating nutraceutical benefits.