What question did this study set out to answer?

Investigate the effects of guanidinium and hydrophobic side chains on gene delivery using polyphosphoesters.

Dual‐Functional Polyphosphoesters for Gene Delivery: Synergistic Effects of Guanidinium and Hydrophobic Side Chains in Degradable Polymers

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Formulating functionalized polymers improves gene transfection in nanoparticles, highlighting chemical properties' impact.

Investigate the effects of guanidinium and hydrophobic side chains on gene delivery using polyphosphoesters.
Synthesized guanidinium- and indole-functionalized PPEs using living anionic ring-opening polymerization and thiol-ene modification
Characterized polyplexes formed with plasmid DNA measuring < 200 nm with zeta potentials of +34 to +43 mV
Evaluated transfection efficiency at varying guanidinium and indole ratios in vitro.
Achieved transfection enhancement up to 200-fold compared to variants with lower charge densities
PPEs demonstrated pH-responsive degradation facilitating controlled DNA release
Exhibited comparable cytocompatibility to linear poly(ethylene imine).

Kötzsche et al. (2025) studied this question.

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