What question did this study set out to answer?

This research aims to optimize gene delivery by balancing cationic, hydrophobic, and pH-responsive components in polymeric carriers.

November 13, 2025International Journal of PharmaceuticsOpen Access

Controlled design of guanidinium-functionalized methacrylamides: tuning polyplex formation and transfection efficiency through hydrophobic and pH-responsive motifs

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Authors

JEJan Egger MKMarkus KötzscheFriedrich Schiller University Jena NTNazita Tavazohi

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Implication

Controlled design of polymeric gene carriers enhances complex stability and transfection efficiency in HEK293T cells, indicating the impact of hydrophobic and pH-responsive elements.

Key Points

This research aims to optimize gene delivery by balancing cationic, hydrophobic, and pH-responsive components in polymeric carriers.
Synthesis of statistical copolymers using aqueous RAFT polymerization
Incorporation of functional monomers like guanidinium and imidazole
Evaluation of polyplex formation, stability, and transfection efficiency in HEK293T cells
Stable nanosized polyplexes (90-130 nm) were formed with high positive charges
Increased addition of guanidinium improved stability and protection against DNase I
The highest transfection efficiency was ∼55% in HEK293T cells with copolymers rich in imidazole and hydrophobic elements.

Cite This Study

Egger et al. (2025) studied this question.

synapsesocial.com/papers/692523c1c0ce034ddc354c45 https://doi.org/10.1016/j.ijpharm.2025.126385

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Implication

Controlled design of polymeric gene carriers enhances complex stability and transfection efficiency in HEK293T cells, indicating the impact of hydrophobic and pH-responsive elements.

Controlled design of guanidinium-functionalized methacrylamides: tuning polyplex formation and transfection efficiency through hydrophobic and pH-responsive motifs

Discussion

Member takes

Implication

Key Points

Cite This Study

Controlled design of guanidinium-functionalized methacrylamides: tuning polyplex formation and transfection efficiency through hydrophobic and pH-responsive motifs

Discussion

Member takes

Implication

Key Points

Cite This Study

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