What question did this study set out to answer?

To develop and evaluate a pH-sensitive, zwitterionic-shielded core-shell polyplex system that overcomes the high cytotoxicity of cationic gene delivery vectors while maintaining high transfection efficiency.

February 2, 2017Langmuir

A Zwitterionic-Shielded Carrier with pH-Modulated Reversible Self-Assembly for Gene Transfection

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Authors

YSYu‐Ju Shih AVAntoine Venault LTLemmuel L. Tayo

Discussion

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Overview

In vitro evaluation demonstrates improved hemocompatibility and acid-triggered transfection of zwitterionic core-shell polyplexes, indicating potential for safe, pH-responsive gene delivery.

Key Points

To develop and evaluate a pH-sensitive, zwitterionic-shielded core-shell polyplex system that overcomes the high cytotoxicity of cationic gene delivery vectors while maintaining high transfection efficiency.
Engineered core-shell polyplexes containing cationic cores of PEI or PDMAEMA complexed with DNA, enveloped by a zwitterionic PAA-b-PSBMA diblock copolymer shell.
Verified polymer-DNA complexation using gel retardation and ethidium bromide displacement assays.
Evaluated hemocompatibility via hemolysis and plasma clotting time assays, and assessed transfection efficiency at pH 6.5 versus pH 7.0 using β-galactosidase reporter assays alongside zeta potential and particle size characterization.
The zwitterionic PAA-b-PSBMA shell reduced cytotoxicity and improved hemocompatibility, producing lower hemolysis activity and extended plasma clotting times at neutral pH.
Deshielding of the copolymer shell in acidic conditions enabled higher gene transfection efficiency at pH 6.5 compared to pH 7.0 across all tested formulations, supported by acid-favorable zeta potential and particle size profiles.

Cite This Study

Shih et al. (2017) studied this question.

synapsesocial.com/papers/6a9e3d429b2dbee2f936855d https://doi.org/10.1021/acs.langmuir.6b03685

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February 2, 2017Langmuir

A Zwitterionic-Shielded Carrier with pH-Modulated Reversible Self-Assembly for Gene Transfection

View Full Paper

Ask AI

Bookmark

Authors

YSYu‐Ju Shih AVAntoine Venault LTLemmuel L. Tayo

Discussion

Member takes

Overview

In vitro evaluation demonstrates improved hemocompatibility and acid-triggered transfection of zwitterionic core-shell polyplexes, indicating potential for safe, pH-responsive gene delivery.

Key Points

To develop and evaluate a pH-sensitive, zwitterionic-shielded core-shell polyplex system that overcomes the high cytotoxicity of cationic gene delivery vectors while maintaining high transfection efficiency.
Engineered core-shell polyplexes containing cationic cores of PEI or PDMAEMA complexed with DNA, enveloped by a zwitterionic PAA-b-PSBMA diblock copolymer shell.
Verified polymer-DNA complexation using gel retardation and ethidium bromide displacement assays.
Evaluated hemocompatibility via hemolysis and plasma clotting time assays, and assessed transfection efficiency at pH 6.5 versus pH 7.0 using β-galactosidase reporter assays alongside zeta potential and particle size characterization.
The zwitterionic PAA-b-PSBMA shell reduced cytotoxicity and improved hemocompatibility, producing lower hemolysis activity and extended plasma clotting times at neutral pH.
Deshielding of the copolymer shell in acidic conditions enabled higher gene transfection efficiency at pH 6.5 compared to pH 7.0 across all tested formulations, supported by acid-favorable zeta potential and particle size profiles.

Cite This Study

Shih et al. (2017) studied this question.

synapsesocial.com/papers/6a9e3d429b2dbee2f936855d https://doi.org/10.1021/acs.langmuir.6b03685

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