Isolation and characterization of myo inositol from an agricultural waste—rice bran

This study focusses on isolation of myo inositol crystals from rice bran (Oryza sativa. indica, Indian variety) an agricultural by-product, using an acid hydrolysis process optimized through response surface methodology (RSM). The obtained crystals were characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). XRD analysis revealed a well-defined crystalline structure with a dominant diffraction peak at 31.74°, indicating high crystallinity. FTIR spectra verified the presence of characteristic hydroxyl and C-O functional groups of myo inositol. HPLC analysis demonstrated approximately 80% purity. The ¹H-NMR spectroscopy was employed to analyze the structural identity of the isolated myo inositol crystals. The spectrum exhibited the characteristic four signal groups with relative intensities of 1:2:2:1, consistent with the molecular symmetry of myo inositol (C₆H₁₂O₆). Proton assignments based on chemical shifts and coupling patterns (H2-H4-H6-H1-H3-H5) corresponded closely with previously reported spectra, confirming successful isolation of structurally pure myo inositol. This study presents a simple and efficient method for recovering myo inositol from Oryza sativa. indica bran, offering a sustainable approach to valorize agro-industrial waste for potential nutraceutical and pharmaceutical applications.