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December 1, 2025BMC Medical Genomics2 citationsOpen Access

Limited value of Nanopore adaptive sampling in a long-read metagenomic profiling workflow of clinical sputum samples

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JTJanetta TopASAndrii SlyzkyiNHNoud Hermans

Key Points

  • Limited utility of adaptive sampling leads to unresolved issues in pore loss affecting sequencing reliability.
  • A significant factor is the characteristics of the metagenomic sample influencing processing efficiency.
  • Analysis of clinical sputum samples highlights gaps in current long-read metagenomic workflows.
  • Findings emphasize the need for advancements in nanopore sequencing technology for improved outcomes.

Abstract

We conclude that the utility of NAS is often limited by the characteristics of the metagenomic sample studied, and that the factors contributing to pore loss need to be resolved before ONT sequencing can be reliably applied to long-read metagenomics.

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Cite This Study

Top et al. (2025) studied this question.

synapsesocial.com/papers/694028c92d562116f290061ehttps://doi.org/10.1186/s12920-025-02272-8
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