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December 16, 2025Journal of Synchrotron Radiation2 citationsOpen Access

For your eyes too: a new set of images of biological materials from the scientific commissioning of the MOGNO beamline at Sirius

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MCMarcos V. ColaçoTATathiana A. AlvarengaNANathaly Lopes Archilha

Key Points

  • To present the commissioning of the MOGNO beamline and its applications in imaging biological materials.
  • Commissioning experiments performed at the MOGNO beamline of the Sirius synchrotron radiation facility.
  • Utilized phase-contrast imaging and microtomography techniques.
  • Focused on non-destructive analysis for biological specimens.
  • Demonstrated high-resolution imaging capabilities for biological samples.
  • Revealed intricate internal morphologies of a mosquito, reptile, and amphibian embryos.
  • Showcased imaging of an archaeological artefact and a fish fossil bone.

Abstract

The high-energy imaging beamline MOGNO was recently designed, installed and commissioned at the Sirius fourth-generation synchrotron radiation source at the LNLS in Brazil. MOGNO, a micro- and nano-imaging beamline, has as primary source a 3.2 T superbend permanent magnet dipole with a critical energy of 19.15 keV and operates in a cone-beam geometry. The present paper addresses the commissioning experiments in propagation-based phase-contrast imaging and microtomography, revealing high-precision details of a wide range of non-stained biological samples with minimal preparation. We illustrate the potential of non-destructive fast high-resolution microtomographic imaging, particularly for fine anatomical studies of Brazilian biological specimens. Three-dimensional investigations reveal the internal morphology of the head of a dengue fever vector mosquito ( Aedes aegypti ) and of the whole embryo of a reptile ( Brasiliscincus agilis ) and of an amphibian ( Eleutherodactylus cochranae ), as well as the features inherent in an archaeological artefact ( Galeocerdo cuvier tooth) and a fish fossil bone (Elopomorpha incertae sedis ).

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Cite This Study

Colaço et al. (2025) studied this question.

synapsesocial.com/papers/6940acc83507a57a7f7a4442https://doi.org/10.1107/s1600577525009993
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