Association of the rs1333048 polymorphism in the ANRIL gene with different clinical forms of periodontitis: A meta-analysis

BACKGROUND Periodontitis is a chronic inflammatory disorder influenced by both behavioral and genetic factors. Among epigenetic regulators, the ANRIL gene has been proposed as a risk factor for periodontitis; however, findings on the association between ANRIL polymorphisms and disease susceptibility remain inconsistent. AIM To analyze the association between the rs1333048 genetic polymorphism in the ANRIL gene and periodontitis via meta-analysis. METHODS A literature search was performed for studies published before May 2, 2025. The Review Manager statistical program was used in analyses with calculations of heterogeneity index (I ²) and odds ratio (OR) with 95% of confidence intervals (CI). Begg’s test and the Egger’s linear regression test were used for publication bias evaluation using Comprehensive meta-analysis software. P < 0.05 was considered significant. RESULTS From 12 studies including 5489 participants across multiple ethnic groups, we observed a statistically significant association between ANRIL gene polymorphisms and periodontitis in the allelic contrast model (OR = 1.24 95%CI: 1.15-1.34, P < 0.00001). Conversely, the wild-type allele was significantly associated with the control group (OR = 0.80 95%CI: 0.75-0.87, P < 0.00001). Heterogeneity was low (I ² = 28%, P heterogeneity = 0.17), and no significant risk of publication bias was detected (P > 0.05). CONCLUSION In conclusion, this meta-analysis demonstrated a significant association between the rs1333048 polymorphism and periodontitis in the overall analysis and in stratified analyses of Caucasian populations, but in for mixed-race populations.