miR-155 suppresses angiotensin II type 1 receptor synthesis during placental morphogenesis

Fig. 1. Fetal and placental weights from miR-155-/- and miR-155+/+ mice at pc day 18.5. A Fetal and (B) placental weights, and C fetal to placental weight ratios in miR-155-/- and miR-155+/+ (control) groups. Data are represented as mean ± SEM. nmiR-155(+/+) = 18 litters with 4–12 fetuses/placentae each to a total of 96. nmiR-155(-/-) = 18 litters with 1–5 fetuses/placentae each to a total of 86. *indicates a significant difference from controls (p < 0.05) as analyzed using a linear mixed model with random intercept accounting for maternal familial correlation.

Fig. 2. Morphological analyses of placentae from miR-155-/- and miR-155+/+ mice at pc day 18.5. A Labyrinth zone area, B labyrinth zone to placental area ratio, C junctional zone area, D junctional zone to placental area ratio and E labyrinth zone to junctional zone area in placentae miR-155-/- and control mice. F Labyrinth zone (LZ) compared with junctional zone (JZ) from the placenta of a control dam and G from the placenta of a miR-155-/- dam. H Magnified (40X objective) images depicting trophoblast cell morphology and spiral artery remodeling in the placenta of a control dam and I from the placenta of a miR-155-/- dam. Data are represented as mean ± SEM. nmiR-155(+/+) = 18 litters with 4–12 placentae each to a total of 96 (all placentae). nmiR-155(-/-) = 18 litters with 1–5 placentae each to a total of 86. * indicates a statistically significant difference from controls (p < 0.05).

Fig. 3. Expression of miR-155 and Agtr1 mRNA and AT1R protein in placentae from miR-155-/- and control mice. A Expression of miR-155, B Agtr1 mRNA and C AT1R protein levels in placentae from miR-155-/- and control mice. Data are represented as mean ± SEM. n = 4–9 placentae/dam for ten dams total. * indicates a statistically significant difference to controls (p < 0.05).

Fig. 4. Abundance of miR-155 and AGTR1 mRNA, and the rate of cell proliferation in miR155 mimic-treated and vehicle-treated HTR-8/SVneo cells. A miR-155 and B AGTR1 mRNA expression in miR-155 mimic-treated and vehicle-treated HTR-8/SVneo cells. C The rate of proliferation, D migration and E invasion of HTR-8/SVneo cells transfected with different doses of a miR-155 mimic. The slopes of the lines indicate HTR8/SVneo cell proliferation, migration and invasion, respectively, over 72 h. Control (red), 7.5 pM mimic (blue), 15 pM mimic (green), 30 pM mimic (magenta), 60 pM mimic (cyan). Graphs D, E include an uncoated serum-free control (black); graph E includes an uncoated control (purple). Data are presented as mean ± SEM. n = 3 experiments, each in triplicate. * indicates a statistically significant difference to the vehicle control (p < 0.05). The same letter above the bars indicates that groups are not different from each other. A different letter above the bars indicates that groups are different (all p < 0.05).