Regulation of the human placental (pro)renin receptor-prorenin-angiotensin system by microRNAs

Human placentae collected in early (10-11 weeks; n=7), mid-gestation (14-18 weeks; n=8), and term (38-40 weeks; n=8) pregnancies, as well as placentae from women with early (29-35.1 weeks; n=8) or late-onset pre-eclampsia (>34-weeks gestation; n=8) and gestational age matched preterm (31.6-35.1 weeks; n=8) and term normotensive controls. Human HTR-8/SVneo trophoblast cells were used for in vitro experiments.

Transfection of miR-181a-5p and miR-663 into human HTR-8/SVneo trophoblast cells.

Term placentae (for gestational age comparisons), normotensive controls (for pre-eclampsia comparisons), and untransfected/control cells (for in vitro experiments).

Differential expression of miRNAs targeting the renin-angiotensin system (RAS) and their effect on placental REN mRNA expression and prorenin protein levels.surrogate