Translating the green code: Ribo-Seq for photosynthetic eukaryotes in focus

Abstract Because they are continually exposed to fluctuating environments, photosynthetic organisms, including land plants and algae, must adapt to a wide range of environmental conditions. For this purpose, translational control plays a pivotal role. The advent of ribosome profiling, or Ribo-Seq, has helped overcome the technical barriers faced by earlier methods, enabling comprehensive and quantitative analysis of protein synthesis. This review highlights recent advances in green lineage Ribo-Seq, covering technical innovations, diverse applications, and analytical insights. These findings emphasize the power and versatility of this technique in exploring translational regulation in photosynthetic species.