A diagnostic method demonstrates effective and portable detection of Theileria annulata in cattle, highlighting practical solutions for cold chain challenges.
AbstractA study was undertaken to develop a paper-based colorimetric loop-mediated isothermal amplification (LAMP) assay for the diagnosis of bovine tropical theileriosis. The hydroxy naphthol blue (HNB) wet LAMP assay was standardized for diagnosis of Theileria annulata in cattle. Further, the optimized wet LAMP assay was performed on a suitable paper substrate for the standardization of HNB paper LAMP with required modifications. The drying of colorimetric LAMP reagents on paper substrate allowed the transport of the ready-to-use LAMP kits to areas where the cold chain is not available and it can be employed for point-of-care diagnostics because of its single step operation.
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Sandeep et al. (2024) studied this question.
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