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January 1, 2025ParasiteOpen Access

Development and validation of a real-time SYBR green PCR method for the detection and differentiation of Babesia and Theileria species (Apicomplexa: Piroplasmida) in hard ticks and cattle blood from Thailand

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Authors

MKMay Thazin KyawPJPenchom JanwanTTTongjit Thanchomnang

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Overview

The real-time PCR assay detects Babesia and Theileria species in cattle blood and ticks, suggesting effective disease monitoring.

Key Points

  • The assay accurately distinguishes between Babesia and Theileria species using specific melting temperatures.
  • Detection rates showed Theileria sinensis at 25.9% in blood samples and B. bigemina at 0.7% in ticks, emphasizing pathogen prevalence.
  • Developed using mitochondrial cytochrome b for Babesia and 18S rRNA genes for Theileria, ensuring specificity.
  • This assay provides a reliable tool for monitoring tick-borne diseases, aiding in agricultural interventions.

Cite This Study

Kyaw et al. (2025) studied this question.

synapsesocial.com/papers/68af6216ad7bf08b1eae385chttps://doi.org/10.1051/parasite/2025040
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Also Consider

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  1. 1Livestock owners’ worry and fear of tick-borne diseases2020 · 28 citations
  2. 2Molecular characterization and genetic diversity of Babesia bovis and Babesia bigemina of cattle in Thailand2022 · 17 citations
  3. 3Development and Validation of a Quantitative PCR Assay Using Multiplexed Hydrolysis Probes for Detection and Quantification of Theileria orientalis Isolates and Differentiation of Clinically Relevant Subtypes2015 · 65 citations
  4. 4Survey of Benign Theileria Parasites of Cattle and Buffaloes in Thailand using Allele-Specific Polymerase Chain Reaction of Major Piroplasm Surface Protein Gene.2003 · 38 citations
  5. 5Molecular Identification of Babesia and Theileria Infections in Livestock in the Qinghai–Tibetan Plateau Area, China2024 · 6 citations