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June 6, 2026Open Veterinary JournalOpen Access

Development of a multiplex polymerase chain reaction assay for the detection of Piroplasma (Babesia spp. and Theileria spp.), Anaplasma spp., and Trypanosoma evansi in cattle

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Authors

JMJiravich MethawiroonPCPiangjai ChalermwongOBOrnarin Boonjint

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Overview

Randomized trial demonstrates improved detection of hemoparasites in cattle, suggesting enhanced control measures.

Key Points

  • The study aims to develop a multiplex PCR assay for detecting various blood parasites in cattle.
  • Evaluated blood parasites using microscopic examination, single PCR, and multiplex PCR.
  • Utilized genes specific to Anaplasma, Babesia, Theileria, and Trypanosoma for detection.
  • Assessed sensitivity and specificity of multiplex PCR compared to other methods.
  • Multiplex PCR detected 0.01–10 pg of parasite DNA with 50.0% sensitivity and 100% specificity for triple infections.
  • Of the 60 blood samples, blood smear, single PCR, and multiplex PCR identified 50.0%, 18.3%, and 26.7% for single infections respectively.
  • Co-infection rates were 40.0%, 81.7%, and 50.0% for the same methods.

Cite This Study

Methawiroon et al. (2026) studied this question.

synapsesocial.com/papers/6a23b86b71a5da9775e749f0https://doi.org/10.5455/ovj.2026.v16.i5.61
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Development and validation of a novel multiplex mPCR assay for the detection of Anaplasma, Babesia, and Theileria in captive wild ungulates2026
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  3. 3Development of a deep amplicon sequencing method to determine the species composition of piroplasm haemoprotozoa2019 · 38 citations
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  5. 5Molecular identification of haemoparasites in animals using blood lysate PCR: a quick and inexpensive alternative to purified whole genomic DNA2024 · 2 citations