What does this research mean for the field?

Piroplasmid infections, including Babesia and Theileria species, are highly prevalent in cattle in Jiri, Nepal, with molecular detection methods demonstrating significantly higher sensitivity (77.5%) compared to microscopic examination (23.8%). Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To evaluate the prevalence and genetic diversity of piroplasmid infections in cattle using both microscopic and molecular diagnostic techniques.

August 22, 2026Parasitology ResearchOpen Access

Molecular detection and diversity of piroplasmids in cattle from Jiri, Nepal

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Authors

MPMadhav Pandey MCMoni Kumari Chaudhary DDDina Nath Dhakal

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Overview

Observational study reveals high piroplasmid infection rates and genetic diversity in cattle, highlighting the necessity of sensitive molecular diagnostics.

Key Points

To evaluate the prevalence and genetic diversity of piroplasmid infections in cattle using both microscopic and molecular diagnostic techniques.
Collected blood samples from cattle (N=80) in Jiri, Nepal for microscopic smear examination and hematological profiling.
Assayed samples using nested PCR (nPCR) targeting the 18S rRNA gene, followed by direct sequencing, cloning (n=27), and phylogenetic analysis.
Nested PCR detected piroplasmids in 77.5% (62/80) of cattle blood samples, compared to 23.8% (19/80) detected by microscopic examination.
Sequencing identified Babesia bovis, Babesia bigemina, Theileria annulata, and Theileria orientalis, with 85.2% (23/27) single infections and 11.1% (3/27) mixed infections.
Hematological analysis revealed no statistically significant correlation between piroplasmid presence and blood parameter alterations.

Cite This Study

Pandey et al. (2026) studied this question.

synapsesocial.com/papers/6a895f53ca7ade938187dde8 https://doi.org/10.1007/s00436-026-08749-4

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August 22, 2026Parasitology ResearchOpen Access

Molecular detection and diversity of piroplasmids in cattle from Jiri, Nepal

View Full Paper

Ask AI

Bookmark

Authors

MPMadhav Pandey MCMoni Kumari Chaudhary DDDina Nath Dhakal

Discussion

Member takes

Overview

Observational study reveals high piroplasmid infection rates and genetic diversity in cattle, highlighting the necessity of sensitive molecular diagnostics.

Key Points

To evaluate the prevalence and genetic diversity of piroplasmid infections in cattle using both microscopic and molecular diagnostic techniques.
Collected blood samples from cattle (N=80) in Jiri, Nepal for microscopic smear examination and hematological profiling.
Assayed samples using nested PCR (nPCR) targeting the 18S rRNA gene, followed by direct sequencing, cloning (n=27), and phylogenetic analysis.
Nested PCR detected piroplasmids in 77.5% (62/80) of cattle blood samples, compared to 23.8% (19/80) detected by microscopic examination.
Sequencing identified Babesia bovis, Babesia bigemina, Theileria annulata, and Theileria orientalis, with 85.2% (23/27) single infections and 11.1% (3/27) mixed infections.
Hematological analysis revealed no statistically significant correlation between piroplasmid presence and blood parameter alterations.

Cite This Study

Pandey et al. (2026) studied this question.

synapsesocial.com/papers/6a895f53ca7ade938187dde8 https://doi.org/10.1007/s00436-026-08749-4

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