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April 28, 2026SHILAP Revista de lepidopterologíaOpen Access

Development and validation of a novel multiplex mPCR assay for the detection of Anaplasma, Babesia, and Theileria in captive wild ungulates

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Authors

NINazish IqrarBABushra AnwarWSWasim Shehzad

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Overview

Randomized trial develops assay detecting hemoparasites in wild ungulates, highlighting parasite prevalence.

Key Points

  • To develop and validate a multiplex PCR assay for detecting hemoparasites in wild ungulates.
  • Developed multiplex PCR assay using specific primers for Anaplasma, Babesia, and Theileria.
  • Collected blood samples from 60 captive wild ungulates from various zoos and wildlife parks.
  • Evaluated samples using PCR and confirmed results with downstream sequencing.
  • 40 out of 60 (66.66%) samples tested positive for hemoparasitic infections.
  • Infection rates were 25% for Anaplasma, 0% for Babesia, and 40% for Theileria.
  • Multiplex PCR results were consistent with monoplex PCR findings.

Cite This Study

Iqrar et al. (2026) studied this question.

synapsesocial.com/papers/69f04d9f727298f751e71f7fhttps://doi.org/10.3389/fitd.2026.1771416
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