What question did this study set out to answer?

The research aims to explore the molecular mechanisms of sesquiterpenoid biosynthesis in Atractylodes macrocephala.

Full‐Length Transcriptome Analysis of Different Origins and Functional Characterization of Terpene Synthase in Atractylodes macrocephala Koidz

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Transcriptome analysis identifies terpene synthase genes in Atractylodes macrocephala, suggesting pathways for enhancing sesquiterpenoid production.

The research aims to explore the molecular mechanisms of sesquiterpenoid biosynthesis in Atractylodes macrocephala.
Utilized next-generation sequencing and SMRT sequencing for transcriptome analysis.
Identified differentially expressed genes in rhizomes from various geographical origins.
Characterized nine terpene synthase genes through sequence analysis and enzymatic assays.
Identified 17,846 differentially expressed genes, with 5,196 upregulated and 12,650 downregulated.
AmTPS8 and AmTPS9 were classified into the TPS-a subfamily, showing distinct expression patterns.
Demonstrated that AmTPS8 produces bulnesol, while AmTPS9 functions as a juniper camphor synthase.

Li et al. (2026) studied this question.

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Transcriptome analysis identifies terpene synthase genes in Atractylodes macrocephala, suggesting pathways for enhancing sesquiterpenoid production.