Randomized trial shows mechanistic insights into terpene biosynthesis in Leucosceptrum canum, indicating potential for sustainable production of sesquiterpenoids.
Key Points
The study aims to characterize six sesquiterpene synthases from Leucosceptrum canum to understand their functional diversity and biosynthetic mechanisms.
Functionally characterized six sesquiTPSs through heterologous expression in Escherichia coli.
Utilized GC-MS and NMR for product identification, alongside phylogenetic analysis and gene expression profiling.
Applied metabolic engineering strategies to establish high-yield production systems for the targeted sesquiterpenoids.
Generated various sesquiterpenoids including germacrene A and (+)-β-himachalene from LcTPS3-LcTPS8.
Phylogenetic analysis revealed evolutionary divergence with classification into TPS-a and TPS-b subfamilies.
Established production systems achieving up to 618.73 mg L⁻¹ of six sesquiterpenoids.