What question did this study set out to answer?

This research aims to enhance tomato regeneration and genetic transformation efficiencies through chemical treatment and gene modulation.

January 23, 2026Open Access

Enhancing tomato regeneration and genetic transformation efficiencies via exogenous chemical treatment and the GRF5 gene modulation

Key Points

This research aims to enhance tomato regeneration and genetic transformation efficiencies through chemical treatment and gene modulation.
Utilized Driver and Kuniyuki Walnut Medium for tomato tissue culture
Supplemented the medium with phloroglucinol
Conducted heterologous expression of the GRF5 gene from Arabidopsis in tomato plants
Notable increase in regeneration efficiency with phloroglucinol treatment
GRF5 expression further improved transformation efficiency
Synergistic effects were observed between phloroglucinol and GRF-mediated pathways

Abstract

Supplementation of Driver and Kuniyuki Walnut Medium with phloroglucinol enhanced regeneration efficiency in tomato tissue culture. Heterologous expression of an Arabidopsis growth-regulating factor gene, GROWTH-REGULATING FACTOR5 (GRF5), in tomato improved regeneration and transformation efficiency, suggesting a synergistic effect between phloroglucinol treatment and GRF-mediated pathways.

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