Cognatiyoonia denitrificans sp. nov., isolated from seawater

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated as R2331 T , was isolated from coastal seawater of Yantai, P.R. China (127.59° E, 37.46° N). The organism grew optimally at 28–32 °C, at pH 7.0 and in the presence of 3.0% (w/v) NaCl. Strain R2331 T contained ubiquinone-10 as the predominant respiratory quinone and C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c as the major fatty acids. The predominant polar lipids of strain R2331 T were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and two unidentified aminolipids. The DNA G+C content of strain R2331 T was 59.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Yoonia ponticola KCTC 42133 T with 96.3% sequence similarity. Calculated ANI values, dDDH values and AAI values between strain R2331 T and the phylogenetically related species were in the range 71.4–73.7 %, 18.0–19.4 % and 67.2–71.1 %, respectively. Strain R2331 T fell within the cluster of the genus Cognatiyoonia in the phylogenomic tree. Based on polyphasic analyses, strain R2331 T represents a novel species of the genus Cognatiyoonia , for which the name Cognatiyoonia denitrificans sp. nov. is proposed. The type strain is R2331 T (=KCTC 8319 T =MCCC 1K04209 T ).