Preparation and efficacy validation of dihydromyricetin nanoliposomes.

Introduction: Dihydromyricetin (DMY), a phytoflavonoid with diverse pharmacological activities, is limited in cosmetic applications by poor solubility, easy discoloration, and low bioavailability. This study aimed to address these drawbacks for its practical cosmetic use. Methods: DMY nanoliposomes (DMY-NL) were prepared via ethanol injection-high-pressure homogenization, with orthogonal tests optimizing the process using particle size, PDI, and zeta potential as indices. Tests included in vitro dialysis-based sustained-release assay, erythrocyte hemolysis/human patch tests (safety), and DPPH scavenging/hemolysis/human patch antiirritation tests (soothing efficacy). Results: DMY-NL had >90% encapsulation efficiency, 90-day stability under different storage conditions, and 48-h sustained release (superior to control). Safety was confirmed by hemolysis and patch tests; soothing efficacy was verified via DPPH scavenging and anti-irritation tests. Discussion: DMY-NL’s high encapsulation, good stability, and sustained release solve DMY’s cosmetic application limitations. Confirmed safety and soothing effects support its practical use in cosmetics. Conclusion: This study provides a theoretical and practical basis for DMY’s cosmetic application, expected to expand its use in the cosmetic industry.