This review highlights tissue culture to explore plant stress responses, indicating its role in improving crop resilience.
Plant stress, driven by environmental factors such as drought, salinity, and temperature extremes, poses significant challenges to global agriculture, affecting food security and biodiversity. Research into plant stress mechanisms is critical for developing resilient crop varieties capable of withstanding such adverse conditions. Tissue culture, a powerful tool in plant biotechnology, offers a unique platform to study plant responses at the cellular and molecular levels. By enabling the propagation of plant cells in controlled environments, tissue culture facilitates the investigation of stress-induced changes in plant physiology, biochemistry, and gene expression. This review aims to highlight the potential of tissue culture techniques in advancing our understanding of plant stress mechanisms, emphasizing their role in exploring cellular responses to stressors, identifying stress-tolerant genotypes, and screening for novel stress-related genes. Key findings suggest that tissue culture enables rapid assessment of plant stress responses, providing insights into stress adaptation pathways, and facilitating the development of stress-tolerant crops. Looking ahead, the integration of tissue culture with modern genomic and transcriptomic tools holds great promise for accelerating the identification of key stress-related genes and pathways, ultimately contributing to the development of more sustainable agricultural practices and improved crop resilience in the face of climate change.
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Mutheeswaran Subramanian*1, Avin Jose1, Vasanth Nayagam2, Madalaimuthu Anthony Samy2, Jesuraj Mariyaraj3, Ramar Govindaraj1 (2026) studied this question.
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