What question did this study set out to answer?

To investigate lipidomic changes in milk due to negative energy balance in transition dairy cows and identify associated biomarkers.

February 2, 2026MetabolitesOpen Access

Negative Energy Balance in Transition Cows Induces Complex Changes in Lipid Profile of Milk

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Examines lipid changes in milk due to negative energy balance in dairy cows, suggesting potential biomarkers for energy status.

To investigate lipidomic changes in milk due to negative energy balance in transition dairy cows and identify associated biomarkers.
Lipid composition analyzed using GC-FID, HILIC-MS, and RP-LC-MS techniques.
Compared milk samples from early lactating (5–14 days in milk) and mid-lactating (65–80 days in milk) cows.
Thorough assessment of various lipid classes in collected milk samples.
Negative energy balance led to significant alterations in all lipid classes examined.
Certain lipid classes, such as triglycerides and phospholipids, showed varied responses, notably with long-chain fatty acids upregulated.
Identified nine specific lipids as potential biomarkers of negative energy balance in dairy cows.

Liu et al. (2026) studied this question.

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