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This research aims to explore the expression and functional role of MKRN1 in renal angiomyolipomas (AMLs).

February 2, 2026Kidney DiseasesOpen Access

Makorin ring finger protein 1 inhibits cell proliferation in renal angiomyolipoma via the ERK/MAPK signaling pathway

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Investigates MKRN1's role in inhibiting cell proliferation in renal AML, suggesting potential diagnostic benefits.

This research aims to explore the expression and functional role of MKRN1 in renal angiomyolipomas (AMLs).
Evaluated MKRN1 expression in AML tissues using immunohistochemistry, western blotting, and quantitative real-time PCR.
Overexpressed MKRN1 in AML cells and assessed cell proliferation with the Cell Counting Kit-8 assay.
Confirmed proliferative activity using immunofluorescence staining of Ki-67.
Performed gene set enrichment analysis (GSEA) on RNA sequencing data and validated pathways with western blotting.
MKRN1 expression was significantly lower in renal AML tissues compared to para-tumorous tissues (p<0.0001).
MKRN1 overexpression notably inhibited survival and proliferation of SV7 and UMB cells.
GSEA and western blotting indicated that MKRN1 downregulates the ERK/MAPK signaling pathway.
Correlations showed MKRN1 expression negatively correlates with elevated phosphorylated ERK (p-ERK) levels in clinical AML samples.

CHANG et al. (2026) studied this question.

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