Analysis of 6‐Gingerol by Using Densitometric High‐Performance Thin‐Layer Chromatography (HPTLC) in Zingiber rubens Roxb. Methanol Extract Collected From Different Locations of Meghalaya

Zingiber rubens Roxb. is a vastly persuasive species of ginger and is an indigenous cultivar native to Northeast India, especially Meghalaya, locally known as “Sying Makhir.” To determine the amount of 6‐gingerol in the methanol extract of Z. rubens collected from different locations of Meghalaya, a sensitive and precise high‐performance thin‐layer chromatography (HPTLC) method has been developed and validated by the International Council for Harmonization (ICH) guidelines. To achieve effective separation, thin‐layer chromatography (TLC) plates precoated with silica gel 60F 254 as the stationary phase and a mobile phase comprising toluene: ethyl acetate: formic acid 8:1.5:0.5 ( v/v/v ) were used. 6‐gingerol had a retention factor ( R F ) of approximately 0.32 ± 0.02 with r 2 = 0.997299% ± 0.10%. The linear regression analysis data for the 6‐gingerol calibration curve demonstrated a satisfactory linearity relationship. 6‐Gingerol was present in all samples in comparable contents, ranging from 28.71 ± 0.05 to 31.32 ± 0.08 µg/mg. The developed method was specific and robust, making it a potentially useful method for the quality control of different plants/formulations containing 6‐gingerol.