Key result
Pomegranate peel extract lowers MMC-induced AST elevation by ~11% and attenuates tissue damage in mice.
Why the study?
Mitomycin-C is a chemotherapeutic agent with serious side effects, and antioxidant-rich pomegranate peel extract may have protective health benefits against this toxicity.
Does pomegranate peel extract prevent Mitomycin-C induced toxicity in female albino mice?
Does pomegranate peel extract prevent Mitomycin-C induced toxicity in female albino mice?
Effect estimate: AST levels increased significantly in MMC group (124.00 U/L) compared to control (77.33 U/L) and decreased to 109.17 U/L (MMC + 150 mg/kg Pomegranate extract) and 110.00 U/L (MMC + 300 mg/kg Pomegranate extract)
Absolute Event Rate: 109.17% vs 77.33%
p-value: p=<0.001
Pomegranate peel extract significantly attenuated Mitomycin-C induced hepatotoxicity and tissue damage in the small intestine and spleen of mice.
Hypothesis-generating for mitigating chemotherapy toxicity; leaves open any human translation or practice change.
Mitomycin-C (MMC) is a chemotherapeutic agent used in cancer treatment but has serious side effects. Pomegranate (Punica granatum L.) peel is rich in antioxidants, phytochemicals, and other bioactive compounds, suggesting potential health benefits. This study aims to evaluate the potential protective effects of pomegranate peel extract against MMC-induced toxicity using spectrophotometric, electrophoretic, histological and image processing methods. In the study, 48 female albino mice (Mus musculus) were divided into six groups and the groups were determined as control, Mitomycin-C (MMC), 150 mg/kg pomegranate peel extract, 300 mg/kg pomegranate peel extract, MMC + 150 mg/kg pomegranate peel extract and MMC + 300 mg/kg pomegranate peel extract. Following the application, blood samples were collected from the mice to determine serum biochemical parameters using spectrophotometric methods and to analyze serum protein expressions via electrophoresis. Additionally, tissue samples from the small intestine and spleen were collected for histological examination. Significant increases in AST enzyme levels were observed in the MMC group, while lower AST enzyme levels were recorded in the MMC + pomegranate peel extract groups.In electrophoretic analyses, it was determined that protein expressions increased only in the MMC-treated group, whereas expressions decreased in the pomegranate peel extract-treated groups and the MMC + 150 mg/kg pomegranate peel extract-treated groups. Histological examinations demonstrated that pomegranate peel extract attenuated tissue damage in the small intestine and spleen caused by MMC. Moreover, Our study has beenreinforcedbyexperimentalresultssupported in detailwithvisual data obtainedthroughgraphicalrepresentations of intensitydistributions of gel images, generatedusingimageprocessingmethods in the MATLAB environment.This study indicates that pomegranate peel extract may have protective effects against MMC toxicity. It is proposed that pomegranate peel extract could be utilized to mitigate the side effects of Mitomycin-C during cancer treatment; however, additional research is required to validate these findings.
No takes yet. Share an insight, caveat, or question.
Evren Koç, Pınar Aksu Kılıçle, Özlem Önen, Erhan Uluman, Ali Berkan URAL (2026) studied Female albino mice (Mus musculus) aged 3-4 months with Mitomycin-C induced toxicity (n=48). Pomegranate (Punica granatum L.) peel extract vs. Control (saline) or MMC 2 mg/kg intraperitoneally was evaluated on Serum aspartate aminotransferase (AST) levels indicating liver toxicity and tissue damage assessment in small intestine and spleen (AST levels increased significantly in MMC group (124.00 U/L) compared to control (77.33 U/L) and decreased to 109.17 U/L (MMC + 150 mg/kg Pomegranate extract) and 110.00 U/L (MMC + 300 mg/kg Pomegranate extract), p=<0.001). Pomegranate peel extract at 150 mg/kg and 300 mg/kg reduced MMC-induced elevation of AST enzyme levels from 124.00 to approximately 110 U/L and attenuated tissue damage in small intestine and spleen in female albino mice.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: