What question did this study set out to answer?

Investigate the physicochemical properties, structural characteristics, and protein composition of the hard corona on nanoparticles across varying plasma concentrations relevant to in vitro and in vivo conditions.

February 2, 2011Journal of the American Chemical Society

Physical−Chemical Aspects of Protein Corona: Relevance toin Vitroandin VivoBiological Impacts of Nanoparticles

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Authors

MMMarco P. MonopoliCenter for Interdisciplinary Studies of Molecular Interactions WDWalczyk DorotaCracow University of Technology ACAbigail CampbellUniversity College Dublin

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Implication

In vitro biophysical analysis reveals shifts in nanoparticle hard protein corona composition across plasma levels, highlighting critical challenges for in vitro-to-in vivo translation.

Key Points

Investigate the physicochemical properties, structural characteristics, and protein composition of the hard corona on nanoparticles across varying plasma concentrations relevant to in vitro and in vivo conditions.
Evaluated protein adsorption on sulfonated polystyrene and silica nanoparticles across various plasma concentrations and protein-to-nanoparticle surface area ratios.
Characterized nanoparticle-protein complexes in situ and after isolation using differential centrifugal sedimentation, dynamic light scattering, and zeta-potential measurements.
Quantified hard corona composition and total binding isotherms using one-dimensional SDS-PAGE and electrospray liquid chromatography mass spectrometry.
Demonstrated that both sulfonated polystyrene and silica nanoparticles acquire distinct, durable hard protein coronas that alter their surface charge and aggregation state.
Observed significant evolution in the identity and abundance of hard corona proteins as environment conditions transitioned from low in vitro protein concentrations to physiological in vivo levels.

Cite This Study

Monopoli et al. (2011) studied this question.

synapsesocial.com/papers/698bee9ea9800b5cbd5a7a5b https://doi.org/10.1021/ja107583h

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Implication

In vitro biophysical analysis reveals shifts in nanoparticle hard protein corona composition across plasma levels, highlighting critical challenges for in vitro-to-in vivo translation.

Physical−Chemical Aspects of Protein Corona: Relevance toin Vitroandin VivoBiological Impacts of Nanoparticles

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Member takes

Implication

Key Points

Cite This Study

Physical−Chemical Aspects of Protein Corona: Relevance toin Vitroandin VivoBiological Impacts of Nanoparticles

Discussion

Member takes

Implication

Key Points

Cite This Study

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