Melatonin protected rats from isoproterenol-induced cardiac mitochondrial enzyme and apoptotic protein alterations and aided complete myocardial injury recovery.
This Expression of Concern alerts readers to potential data integrity issues regarding duplicated western blot control bands in a 2012 rat study on melatonin and myocardial injury.
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EXPRESSION OF CONCERN : D. Mukherjee, A. K. Ghosh, A. Bandyopadhyay, et al., “Melatonin Protects Against Isoproterenol‐Induced Alterations in Cardiac Mitochondrial Energy‐Metabolizing Enzymes, Apoptotic Proteins, and Assists in Complete Recovery From Myocardial Injury in Rats,” Journal of Pineal Research 53, no. 2 (2012): 166–179, https://doi.org/10.1111/j.1600-079X.2012.00984.x . This Expression of Concern is for the above article, published online on 20 February 2012 in Wiley Online Library ( wileyonlinelibrary.com ), and has been issued by agreement between the journal Editor‐in‐Chief, Gianluca Tosini; and John Wiley & Sons Ltd. A third party raised concerns that the beta‐actin control bands in Figures 3A, 4A, 4B, 5A, 5B, 6A, 6B, 7A, and 7B were duplicates and had also been used in an earlier article by most of the same authors Mukherjee et al. 2010 ( https://doi.org/10.1111/j.1600-079X.2010.00749.x ). The authors responded to an inquiry by the publisher and stated that the studies reported in both articles were part of a single experimental project conducted between 2008 and 2009, which used identical sample sets processed under uniform conditions. Most of the original data were no longer available. As such, the publisher is not able to validate the data related to these concerns. Additionally, this article did not adequately indicate that both articles derived from the same experimental procedure and did not declare the re‐use of beta‐actin control bands. This Expression of Concern has been published in order to inform and alert readers about these concerns. The authors were informed of this Expression of Concern.
A Wed, study reported a other. Melatonin protected rats from isoproterenol-induced cardiac mitochondrial enzyme and apoptotic protein alterations and aided complete myocardial injury recovery.