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To develop a high-throughput, multiplexed fluorescence imaging technique utilizing polymer dot probes.

BPS2026 – Multiplexed fluorescence imaging using polymer dot spectral unmixing and rapid line-scan acquisition

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To develop a high-throughput, multiplexed fluorescence imaging technique utilizing polymer dot probes.
Leveraged a rapid line-scan spectral microscopy platform with polymer dot probes.
Achieved 8-channel imaging in antibody-labeled mouse kidney sections using a single excitation source.
Incorporated iterative spectral unmixing algorithms and GPU-accelerated processing for improved accuracy.
Successfully expanded multiplexing capacity to achieve multiplexed imaging in biological systems.
Current system offers imaging with 8 distinct channels, with plans to expand to 11 channels.
Significantly reduced processing time for high-throughput imaging.

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Demonstrating high-throughput multiplexed imaging in biological systems using a novel spectral unmixing approach.

Chris Kim (2026) studied this question.

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