A simple and highly sensitive LC-MS/MS bioanalytical method for phosphorodiamidate morpholino oligonucleotides in plasma

Key Points

• To develop a highly sensitive method for determining viltolarsen in plasma using LC-MS/MS techniques.
• Optimized reversed-phase liquid chromatography (RPLC) and protein precipitation (PPT) processes.
• Applied the method to various phosphorodiamidate morpholino oligomers with different sequences.
• Achieved high sensitivity in detecting viltolarsen in plasma.
• Successfully implemented the method on various oligonucleotide sequences.