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This research aims to characterize the role of BA PrkA, a novel AAA+ ATPase, in sporulation and stress responses in Bacillus anthracis.

February 21, 2026Journal of BacteriologyOpen Access

A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis

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Authors

NSNitika Sangwan ABAnkur Bothra APAndrei P. Pomerantsev

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Overview

Findings demonstrate that BA PrkA is crucial for spore development and resilience against osmotic stress in Bacillus anthracis, linking sporulation and stress responses.

Key Points

This research aims to characterize the role of BA PrkA, a novel AAA+ ATPase, in sporulation and stress responses in Bacillus anthracis.
Identified BA PrkA protein through sequencing and homology analysis with Bacillus subtilis PrkA.
Conducted expression experiments to assess protein timing during sporulation stages II to VI.
Developed and analyzed a null mutant lacking BA PrkA to evaluate sporulation and stress response.
Performed interactome analysis to identify potential protein partners involved in stress response and sporulation.
BA PrkA is essential for sporulation, with null mutants showing severe defects and reduced spore viability.
Null mutants display compromised growth under ionic-osmotic stress, indicating stress sensitivity.
BA prkA is significantly induced in wild-type B. anthracis under osmotic stress conditions.
Interaction analyses identified ProA and EzrA as proteins involved in osmotic stress and sporulation processes.

Cite This Study

Sangwan et al. (2026) studied this question.

synapsesocial.com/papers/69994c80873532290d0210ee https://doi.org/10.1128/jb.00518-25

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February 21, 2026Journal of BacteriologyOpen Access

A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis

View Full Paper

Ask AI

Bookmark

Authors

NSNitika Sangwan ABAnkur Bothra APAndrei P. Pomerantsev

Discussion

Member takes

Overview

Findings demonstrate that BA PrkA is crucial for spore development and resilience against osmotic stress in Bacillus anthracis, linking sporulation and stress responses.

Key Points

This research aims to characterize the role of BA PrkA, a novel AAA+ ATPase, in sporulation and stress responses in Bacillus anthracis.
Identified BA PrkA protein through sequencing and homology analysis with Bacillus subtilis PrkA.
Conducted expression experiments to assess protein timing during sporulation stages II to VI.
Developed and analyzed a null mutant lacking BA PrkA to evaluate sporulation and stress response.
Performed interactome analysis to identify potential protein partners involved in stress response and sporulation.
BA PrkA is essential for sporulation, with null mutants showing severe defects and reduced spore viability.
Null mutants display compromised growth under ionic-osmotic stress, indicating stress sensitivity.
BA prkA is significantly induced in wild-type B. anthracis under osmotic stress conditions.
Interaction analyses identified ProA and EzrA as proteins involved in osmotic stress and sporulation processes.

Cite This Study

Sangwan et al. (2026) studied this question.

synapsesocial.com/papers/69994c80873532290d0210ee https://doi.org/10.1128/jb.00518-25

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