This research characterizes the impact of irradiation on the blood-brain tumor barrier in diffuse midline glioma, suggesting potential for improved therapies.
Key Points
To investigate the effects of irradiation on the physical and metabolic properties of the blood-brain tumor barrier model in diffuse midline glioma.
Developed an in vitro model of human syngeneic blood-brain tumor barrier (BBTB) using endothelial cells, pericytes, and DMG cells.
Administered a single dose of radiation (2 Gγ to 6 Gγ) and characterized the barrier properties over 7 days post-exposure.
Compared the effects of irradiation on the BBTB model with those on a physiological blood-brain barrier (BBB) model.
Endothelial permeability remained preserved for up to 7 days in both BBB and BBTB models post-irradiation.
Claudin-5 showed heterogeneous distribution and decreased expression in endothelial cells after irradiation.
Irradiation enhanced the physical integrity response of the BBB to TNFα, while the effect on BBTB was less pronounced.
Radiation modulated metabolic properties at the transcriptional level with varying effects in endothelial cells and pericytes.