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February 24, 2026Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)Open Access

Preparation of Incurred Samples Containing Egg-derived Protein and the Pilot Study for Proficiency Test

Mai Hosoya Kana Uchida Shinobu Wakuri Shihomi Kuwano Rie Ito Y. Iwasaki Takaho Watanabe Hiroshi Akiyama

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Key Points

This research aims to evaluate the applicability of real samples for external quality control in allergen testing.
Screened 24 commercial food samples using the ELISA method.
Selected a dressing sample closest to the target egg-derived protein concentration.
Diluted selected sample with a non-egg containing dressing.
Conducted a pilot study with 22 institutions using two assay kits.
The diluted sample showed a mean concentration of 10.4±0.6 μg/g.
Pilot study samples had average concentrations of 11.9 μg/g and 9.7 μg/g for the new sample.
Conventional samples yielded average concentrations of 8.6 μg/g and 9.3 μg/g.
Indications that real samples are suitable for external quality control in allergen testing.

Implication

Pilot study demonstrates reliability of external quality control in allergen testing using real samples.

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Cite This Study

Hosoya et al. (2026) studied this question.

synapsesocial.com/papers/699fe34695ddcd3a253e6fc6 https://doi.org/10.3358/shokueishi.67.16

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Detection of Egg, Milk, Wheat, Buckwheat, and Peanut in FoodsOriginal Research · 2006 · 58 citations
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5Creation of a standardized egg ladder for <scp>IgE</scp> ‐mediated egg allergyOriginal Research · 2026 · 4 citations

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