What question did this study set out to answer?

The aim is to explore novel compounds by genetically dereplicating secondary metabolite biosynthetic pathways in Penicillium expansum.

February 26, 2026Journal of Natural ProductsOpen Access

Genetic Dereplication of Multiple Penicillium expansum Biosynthetic Gene Clusters Reveals Cryptic Penta-Cyclopeptide Production

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Authors

MRMira Syahfriena Amir RawaUniversity of Wisconsin–Madison BHBenjamin J. HaefnerUniversity of Wisconsin–Madison GNGrant NicklesUniversity of Wisconsin–Madison

Discussion

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Implication

Uncovers cryptic penta-cyclopeptide production in Penicillium expansum by deleting major biosynthetic gene clusters, suggesting novel metabolic pathways.

Key Points

The aim is to explore novel compounds by genetically dereplicating secondary metabolite biosynthetic pathways in Penicillium expansum.
Engineered a 'flatline' strain of Penicillium expansum by knocking out key biosynthetic genes.
Conducted comparative metabolomic profiling of flatline and wild-type strains.
Cultivated the strains on rice medium to evaluate cyclopeptide production.
Identified compounds and predicted associated biosynthetic gene clusters using antiSMASH.
Increased production of five-residue cyclopeptides in the flatline strain.
Identified known cyclopeptide MBJ-0110 and its analogues, 3-hydroxy-MBJ-0110 and Val-MBJ-0110.
Deletion of the NRPS gene mbjA abolished production of these cyclopeptides.
High conservation of the mbjA gene across 19 fungal species in the Eurotiomycetes taxon.

Cite This Study

Rawa et al. (2026) studied this question.

synapsesocial.com/papers/699fe34695ddcd3a253e7072 https://doi.org/10.1021/acs.jnatprod.5c01531

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Implication

Uncovers cryptic penta-cyclopeptide production in Penicillium expansum by deleting major biosynthetic gene clusters, suggesting novel metabolic pathways.

Genetic Dereplication of Multiple Penicillium expansum Biosynthetic Gene Clusters Reveals Cryptic Penta-Cyclopeptide Production

Discussion

Member takes

Implication

Key Points

Cite This Study

Genetic Dereplication of Multiple Penicillium expansum Biosynthetic Gene Clusters Reveals Cryptic Penta-Cyclopeptide Production

Discussion

Member takes

Implication

Key Points

Cite This Study

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