What question did this study set out to answer?

The research aims to create a highly sensitive lateral flow immunoassay for detecting albendazole residues using composite nanostructures.

February 27, 2026

Fe 3 O 4 Composite Nanostructures-Enabled Fluorescence Quenching Lateral Flow Immunoassay for Sensitive Detection of Albendazole Residues In Agri-Food Products

Key Points

The research aims to create a highly sensitive lateral flow immunoassay for detecting albendazole residues using composite nanostructures.
Synthesized Fe3O4@Au, Fe3O4@Se, and Fe3O4@MnO2 nanostructures.
Developed dual-mode lateral flow immunoassay platforms.
Measured the detection limit and quenching efficiency of the assays on real samples.
Fe3O4@Au-based LFIAs detected albendazole at 0.072 ng mL-1, 69.4-fold more sensitive than colorimetric mode.
Demonstrated a high molar extinction coefficient of 8.877 × 10^11 M^-1 cm^-1.
Achieved strong quenching efficiency at 92.2% and precision with CV < 13%.

Abstract

This study successfully synthesized Fe3O4@Au, Fe3O4@Se, and Fe3O4@MnO2 nanostructures with inherent oxidation properties of Fe3O4 nanoparticles. These nanostructures exhibited plasmonic properties, facilitating visible light absorption and spectral overlap with quantum dots fluorescent microspheres (QD-FMs) for quenching QD-FMs via an inner filter effect. Based on the composite nanostructures, we developed dual-mode (colorimetric, CM, "turn off" mode; fluorescence quenching, FQ, "turn on" model) LFIA platforms. Among them, the Fe3O4@Au-based FQ-LFIA demonstrated superior sensitivity for albendazole (ABZ) which is a veterinary with a detection limit of 0.072 ng mL-1, 69.4-fold lower than Fe3O4@Au-CM-LFIA (5 ng mL-1). The result is attributed to the high molar extinction coefficient (8.877 × 1011 M-1 cm-1), strong quenching efficiency (η = 92.2%), and high quenching constants (Kn = 67.236) of Fe3O4@Au. The assay also demonstrated satisfactory recovery (80.4-108.6%) and precision (CV 3O4@Au-FQ-LFIA is effective for detecting ABZ in agri-food products.

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Cite This Study

Yin et al. (Wed,) studied this question.

synapsesocial.com/papers/69a134fbed1d949a99abe6ca https://doi.org/https://doi.org/10.1021/acs.jafc.5c12487

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