What question did this study set out to answer?

The study aims to determine the optimal reperfusion interval after tourniquet application to minimize muscle damage and malondialdehyde levels.

February 28, 2026Medicinski Glasnik0 citationsOpen Access

The effect of tourniquet reperfusion interval on malondialdehyde (MDA) level and skeletal muscle damage in the treatment of long bone fractures

AUAdhi Satriyo UtomoUniversity of Brawijaya THThomas Erwin Christian Junus HuwaeRSUD Prof. Dr. Saiful Anwar Malang

Puntos clave

The study aims to determine the optimal reperfusion interval after tourniquet application to minimize muscle damage and malondialdehyde levels.
Induced tibial fractures in 48 male rats for experimental analysis.
Divided rats into two groups with four treatments each, totaling eight groups.
Administered tourniquet for 2 to 3 hours with varying reperfusion intervals of 5, 10, and 15 minutes.
Analyzed malondialdehyde (MDA) levels and muscle histology using one-way ANOVA.
Group A showed significant differences in outcomes between the 10 and 15-minute reperfusion intervals.
Group B had a 42.63% reduction in injury with the 10-minute interval and a 32.27% reduction overall.
Significant differences in MDA levels were observed between control and reperfusion groups in both groups A and B.

Resumen

pstrongAim /strongTourniquets are commonly used during surgery to control bleeding; however, their application can lead to complications, including ischemic reperfusion injury. Post-tourniquet syndromes such as pain, swelling, and muscle weakness may persist for up to six weeks. Aim of this study was to investigate the reperfusion interval duration to prevent muscle tissue damage./p pstrongMethods /strongThis experimental study involved 48 male white rats (emRattus norvegicus/em) with induced tibial fracture. The rats were divided into two groups, A and B, with each group receiving four different treatments, resulting in a total of eight groups. The control groups (A1 and B1) had the tourniquet applied for 3 hours, while other groups had it used for 2 hours, followed by 5, 10, and 15-minute reperfusion intervals. Group A was sacrificed one hour post-deflation, and Group B was sacrificed on day 14. Malondialdehyde (MDA) level and muscle histology were analysed using one-way ANOVA./p pstrongResults /strongIn group A, significant difference was found between the 10 and 15-minute reperfusion intervals. In group B, the 10-minute reperfusion interval showed the most favourable outcome, with a 42.63% reduction in injury for group A and a 32.27% reduction for group B. Significant differences in MDA level was found between the control and reperfusion groups in both groups A and B./p pstrongConclusion /strongThe 10-minute reperfusion interval effectively reduces ischemic reperfusion injury in muscle tissue, as indicated by lower MDA level and less muscle damage. This interval optimally restores aerobic metabolism and prevents excessive reactive oxygen species (ROS) production./p

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