What does this research mean for the field?

The ethanolic extract of Putranjiva roxburghii leaves exhibits dose-dependent anxiolytic effects in rat models, comparable to diazepam. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research investigates the anxiolytic effects of the ethanolic extract of Putranjiva roxburghii leaves in rat models.

March 1, 2026Open Access

Investigation of Anxiolytic Potential of Ethanolic Extract of Putranjiva Roxburghii Wall Leaves in Rat Models

Key Points

This research investigates the anxiolytic effects of the ethanolic extract of Putranjiva roxburghii leaves in rat models.
Prepared ethanolic extract using Soxhlet extraction with a yield of 20.6%
Conducted behavioral tests: Elevated Plus Maze (EPM), Open Field Test (OFT), Head-Dipping Test (HBT), and Rotarod test
Administered different doses of the extract (250 mg/kg and 500 mg/kg) and compared to a standard (diazepam)
Measured monoamine oxidase (MAO) activity and lipid peroxidation post-treatment
High dose (500 mg/kg) improved open arm exploration in the EPM and head-dipping behavior in the HBT compared to control
Both doses increased time spent in the central zone of the OFT and reduced muscle rigidity in the rotarod test
Biochemical analysis showed dose-dependent reductions in MAO activity and lipid peroxidation, indicating antioxidative potential

Abstract

Anxiety disorders, marked by persistent fear and excessive worry, impair daily functioning and quality of life. While conventional anxiolytics are effective, their long-term use often leads to side effects, prompting interest in safer, plant-based alternatives. Putranjiva roxburghii Wall, a traditional medicinal plant, has been used to treat various ailments. This study aimed to investigate the anxiolytic potential of the ethanolic extract of Putranjiva roxburghii wall leaves in rat models through behavioral and biochemical assessments. Methodology: EEPR was prepared using Soxhlet extraction from shade-dried leaves, yielding 20.6%. Phytochemical screening revealed the presence of alkaloids, glycosides, flavonoids, tannins, steroids, saponins, triterpenes, resins, amino acids, and carbohydrates. The study was conducted with four groups of rats: control, standard (diazepam), low dose (250 mg/kg), and high dose (500 mg/kg) EEPR. Behavioral tests were performed weekly, including: EPM: Assessed open arm and center activity. OFT: Evaluated exploratory behavior and locomotor activity. HBT: Measured exploratory behavior through head-dipping. Rotarod test: Monitored motor coordination and spontaneous activity. On the 29th day, biochemical parameters such as monoamine oxidase (MAO) activity and lipid peroxidation were assessed to measure neurochemical effects and oxidative stress. Result: The ethanolic extract of Putranjiva roxburghii leaves (20.6% yield) contained alkaloids, glycosides, flavonoids, tannins, steroids, saponins, and other bioactive compounds. Behavioral tests demonstrated that the high dose (500 mg/kg) significantly improved openarm exploration in the (EPM) and enhanced head-dipping behavior in the (HBT), comparable to diazepam. Both doses increased time spent in the central zone of the (OFT) and reduced muscle rigidity in the rotarod test. Biochemical analysis revealed dose-dependent reductions in MAO activity and lipid peroxidation, highlighting its anxiolytic and antioxidative potential. Conclusion: The EEPR showed dose-dependent anxiolytic effects, supported by behavioral and biochemical improvements. Phytochemicals like alkaloids, glycosides, and flavonoids may underlie its activity, highlighting its potential for further clinical exploration.

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Cite This Study

Rohan K. M.*, Dr. Nataraj G. R., T. A. Noor Mohammed Khadeer (Sun,) studied this question.

synapsesocial.com/papers/69a3d830ec16d51705d2ed07 https://doi.org/https://doi.org/10.5281/zenodo.18797313

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