What question did this study set out to answer?

The aim is to enhance the efficiency of CRISPR/Cas9 for gene editing in cultivated strawberry by increasing transformation rates and reducing regeneration times.

March 7, 2026Plant Physiology and BiochemistryOpen Access

Efficient CRISPR/Cas9 system established via co-cultivation of plantlets and Agrobacterium tumefaciens for positive transgenic calluses generation and regeneration in cultivated strawberry (Fragaria × ananassa)

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Authors

XLXu Li LLLili Liu CLChun Luo

Discussion

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Overview

Agrobacterium-mediated gene editing shows a positive transformation rate in cultivated strawberry, indicating potential for genetic improvements.

Key Points

The aim is to enhance the efficiency of CRISPR/Cas9 for gene editing in cultivated strawberry by increasing transformation rates and reducing regeneration times.
Developed an Agrobacterium tumefaciens-mediated CRISPR/Cas9 system for gene editing.
Designed and cloned sgRNAs targeting strawberry transcription factors.
Co-cultivated plantlets with Agrobacterium for 10 days to induce transformation.
Cultured positive calluses on Murashige and Skoog media for shoot regeneration.
Achieved 61.9% and 68.6% GFP-positive calluses for two target genes after co-cultivation.
Successfully generated 3-5 positive shoots from different calluses after 50-80 days.
All T0 lines for targeted transcription factors were edited at the desired sites with high efficiency.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69abc0de5af8044f7a4e986d https://doi.org/10.1016/j.plaphy.2026.111195

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March 7, 2026Plant Physiology and BiochemistryOpen Access

Efficient CRISPR/Cas9 system established via co-cultivation of plantlets and Agrobacterium tumefaciens for positive transgenic calluses generation and regeneration in cultivated strawberry (Fragaria × ananassa)

View Full Paper

Ask AI

Bookmark

Authors

XLXu Li LLLili Liu CLChun Luo

Discussion

Member takes

Overview

Agrobacterium-mediated gene editing shows a positive transformation rate in cultivated strawberry, indicating potential for genetic improvements.

Key Points

The aim is to enhance the efficiency of CRISPR/Cas9 for gene editing in cultivated strawberry by increasing transformation rates and reducing regeneration times.
Developed an Agrobacterium tumefaciens-mediated CRISPR/Cas9 system for gene editing.
Designed and cloned sgRNAs targeting strawberry transcription factors.
Co-cultivated plantlets with Agrobacterium for 10 days to induce transformation.
Cultured positive calluses on Murashige and Skoog media for shoot regeneration.
Achieved 61.9% and 68.6% GFP-positive calluses for two target genes after co-cultivation.
Successfully generated 3-5 positive shoots from different calluses after 50-80 days.
All T0 lines for targeted transcription factors were edited at the desired sites with high efficiency.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69abc0de5af8044f7a4e986d https://doi.org/10.1016/j.plaphy.2026.111195

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