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April 26, 2026BMC Research NotesOpen Access

Rapid screening of genome edited strawberry (Fragaria ×ananassa) regenerants using high-resolution melting analysis followed by Amplicon sequencing

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Authors

KVKaitlyn VondracekMLMan Bo LeeTLTie Liu

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Overview

Randomized trial developed a rapid method for detecting genome edits in strawberry, suggesting it improves screening efficiency.

Key Points

  • The central aim is to create an efficient workflow for rapid detection and genotyping of genome-edited strawberry lines.
  • Developed a high-throughput workflow using high-resolution melting analysis and amplicon sequencing to screen regenerant plant materials.
  • Focused on identifying CRISPR/Cas9-mediated edits in octoploid strawberry lines.
  • Screening was based on distinct mutation profiles and editing efficiencies among different subgenomes.
  • High-resolution melting analysis reliably identified lines with distinct mutation profiles.
  • Amplicon sequencing confirmed the editing events at targeted sites.
  • Editing efficiencies varied among the subgenomes of the octoploid strawberry.

Cite This Study

Vondracek et al. (2026) studied this question.

synapsesocial.com/papers/69edac9b4a46254e215b45c8https://doi.org/10.1186/s13104-026-07792-9
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