August 13, 2025

Possible Reversion of CRISPR-Cas9-Edited Sequences in Octoploid Strawberry.

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Gene editing reveals potential reversion of mutated sequences in octoploid strawberry, suggesting challenges with high-ploidy materials.

Gene editing in octoploid strawberries is complex due to multiple gene copies, which impacts CRISPR-Cas9 efficacy.
The gene-edited line FaMYB9CR-15 displayed distinct leaf morphologies and gene expression patterns compared to wild-type leaves.
Over time, the edited leaves transitioned from wrinkled to smooth, with a notable decrease in mutant allele presence from 70.2% to 43.7%.
Findings highlight the challenges of maintaining genetic changes in high-ploidy plants, impacting future gene editing efforts.

Sun et al. (2025) studied this question.

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August 13, 2025

View Full Paper

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Authors

Gene editing reveals potential reversion of mutated sequences in octoploid strawberry, suggesting challenges with high-ploidy materials.

Gene editing in octoploid strawberries is complex due to multiple gene copies, which impacts CRISPR-Cas9 efficacy.
The gene-edited line FaMYB9CR-15 displayed distinct leaf morphologies and gene expression patterns compared to wild-type leaves.
Over time, the edited leaves transitioned from wrinkled to smooth, with a notable decrease in mutant allele presence from 70.2% to 43.7%.
Findings highlight the challenges of maintaining genetic changes in high-ploidy plants, impacting future gene editing efforts.

Sun et al. (2025) studied this question.

View Full Paper

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Bookmark