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The aim is to investigate the microbial profile of infected wounds and the antibacterial effects of Citrus sinensis extracts against these bacteria.

March 7, 2026Open Access

Antibacterial Activity of Sweet Orange (Citrus Sinensis) on Bacteria Isolated From Wound Infections

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Observational study evaluates Citrus sinensis extracts' antibacterial effects on wound pathogens, highlighting antibiotic resistance.

The aim is to investigate the microbial profile of infected wounds and the antibacterial effects of Citrus sinensis extracts against these bacteria.
Observational laboratory-based study with 200 patients having infected wounds.
Microbial culture and identification using standard microbiological and biochemical methods.
Assessment of antibacterial activity through agar well diffusion and measurement of inhibition zones.
Minimum inhibitory concentration (MIC) determined using tube dilution method.
Statistical analysis performed with SPSS, significance at P < 0.05.
103 out of 200 wound samples (51.5%) were culture-positive.
Monomicrobial infections were found in 74.76% of cases, with 67.44% being Gram-negative bacteria.
S. aureus was the most common isolate at 23.26%, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa.
C. sinensis juice extract showed stronger antibacterial activity than peel extract with maximum inhibition of 28.00 ± 2.10 mm against S. aureus.
Complete inhibition of S. aureus and E. coli at concentrations ≤10 mg/mL was observed.

Dr. Avinash Kumar Bhatia2 and Dr. Raj Kumar3 Shruti Pandey*1 (2026) studied this question.

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